EzPCRTM BASIC Plus 5X PCR Master Mix is a ready-to-use premix containing all the components essential for a PCR and agarose gel electrophoresis (DNA polymerase, dNTP, reaction buffer, glycerol, bromophenol blue, and stabilizer). PCR can be performed simply by adding primer pair and template. 

  EzPCRTM BASIC Plus 5X PCR Master Mix is amixtureof rTaq DNA polymerase and Pfu DNA Polymerase. rTaq DNA polymerase that is a recombinant thermostable DNA polymerase from Thermus aquaticus has a 5’ à 3’ exonuclease activity. EzPCRTM BASIC Plus 5X PCR Master Mix is recommended for use in conventional PCR process.

  As Master Mix is supplied as a 5X concentration format, users should adjust a final reaction to 1X (if final reaction volume is 20 ㎕, 4 ㎕of 5X Master Mix should be added).

  One unit of rTaq DNA polymerase is contained in 4 ㎕ of 5X Master Mix.

 Performance test, exo/endo nuclease contamination test, stability test. genomic DNA contamination test.

  Store at -20 ℃.

  EzPCR™ BASIC Plus 5X PCR Master Mix is stable for at least 1 years at recommended storage condition

1. Prepare the 20 ㎕ PCR solution as follows

 

  

2. Set PCR cycling as follows

 

 

   * Set 25-35 PCR cycles for effective amplification.