EzPCR™ HS 2X PCR master mix includes all components required for PCR except DNA template and primers. This pre-mixed formulation saves time and reduce contamination. Also, an addition of gel loading buffer is no needed.

   HS Polymerase is a mixture of PCR enzyme derived from rTaq DNA polymerase and monoclonal antibody to Taq DNA polymerase. The antibody inhibits the polymerase activity and prevent non-specific amplification derived from misprinting of primer dimer before PCR cycling. The antibody is denatured during the initial denaturation step, allowing this product to be used with standard PCR. This product provides convenience PCR that enhances the specificity and sensitivity.

   rTaq DNA polymerase that is a recombinant thermostable DNA polymerase from Thermus aquaticus has a 5’ à 3’ exonuclease activity and generates a 3’dA overhang.

  

  HOT-start PCR, RT-PCR, genotyping, SNP PCR, multiplex PCR. 

  Activity, SDS-PAGE purity, performance tests, genomic DNA contamination test, confirmation test for the absence of endo and exonucleases.

  Store at -20 ℃.

  EzPCR™ HS 2x PCR Master Mix is stable for at least 1years at recommended storage condition. 

- 5’ → 3’ exonuclease : yes

- 3’ → 5’ exonuclease : no

- strand displacement : no

 

1. Prepare 20㎕ PCR solution as follows

 

PCR grade distilled water                           -  ㎕                        

EzPCR™ HS 2x PCR Master Mix              10 ㎕

Primer (10 pmol/㎕)                         each 0.5 ㎕     

Template                                             1 – 50 ng    

                                                                                      

Adjust final vol. to 20 ㎕ with PCR grade distilled water.

 

2. Set PCR cycling as follows

Initial denature  at 95℃ : 3min 

     

* Set 25-45 PCR cycles for effective amplification