HS One-step RT-qPCR 2X Master Mix (SYBR green, high ROX)  includes all kind of real-time RT-qPCR components such as Taq DNA polymerase, thermostable M-MLV reverse transcriptase, dNTP, buffer and SYBR Green. 

 It is only needed to perform quantitative RT-qPCR by simply adding total or poly(A) RNA as substrates and specific primer pair to the premix.

 HS One-step RT-qPCR 2X Master Mix (SYBR green, high ROX) has been optimized for highly sensitive and reproducible real-time PCR experiments.

 In addition, Taq polymerase is a mixture of PCR enzyme and monoclonal antibody to Taq DNA polymerase. The antibody inhibits the polymerase activity and prevent non-specific amplification derived from mis-priming of primer dimer before PCR cycling.

  

One-step RT-qPCR (SYBR) 

 - Enhanced specificity by use of HOT start with anti-Taq antibody

 - Includes 50 nM/rxn ROX reference dye

 

  Performance test, exo/endo nuclease contamination test, stability test. 

  

Store at -20 ℃ (stable up to 12 months).

Avoid repeated freeze-thaw cycle.  Keep the light out.